Mix & Go! XJa (DE3) Autolysis Competent Cells Size:10 x 100 µl Atlantis dsDNase is a double-stranded

Atlantis dsDNase is a double-stranded DNA-specific endonuclease that cleaves phosphodiester bonds in DNA to yield homogeneous populations of core nucleosomes

The protocol can be performed manually or using an automated platform for high throughput processing

six 50ml tubes

RNA Pre-Wash Buffer is used for the purification of RNA in the Quick-RNA Midiprep kit

Mix & Go! XJa (DE3) Autolysis Competent Cells Size:10 x 100 µl Atlantis dsDNase is a double-strandedWhile there are many cell lysis methods available to scientists, unfortunately none of these methods combine all the ideal features for simple, efficient, economical, and gentle lysis of E. coli cells. The E. coli XJ autolysing strains from Zymo Research were engineered to address this problem. Mild expression of a chromosomally encoded bacteriophage R gene, encoding the lysozyme, also known as endolysin, is induced during growth. Cells are harvested