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HMGB, High mobility group box

DNA methylation Genomic DNA was isolated from the liver tissue or cultured cells using an AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Germantown, MD) following the manufacturers instructions

Fish feeding procedure and sampling Juvenile large yellow croaker fish with an average initial body weight of 859 g were chosen as the subjects, and 270 healthy fish were randomly distributed into nine floating sea cages (1 m 1 m 25 m)

Furthermore, to clarify the role of L-carnitine in male fertility, in a randomized controlled trail in 2015, 20 couples with idiopathic oligoasthenozoospermia were admitted for treatment with L-carnitine (1000 mg/day ) for 3 to 6 months