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This powerhouse molecule is often hailed as the body's master antioxidant, crucial for detoxification and protecting cells from damage

Significantly, as shown in Figure 6 in their Extended Data ( only to the inactive M pro C145A mutant, and concluded it was a likely M pro substrate, because they were able to identify a putative M pro cleavage site in the TRMT1 protein sequence (PRLQ/ANFT), where the slash (/) represents the cleavage site

Furthermore, inclusion of a His-tag during expression (for subsequent Ni-nitrilotriacetic acid purification) might alter protein folding and thus binding results (Ledent et al., Table 2)