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Blood samples were immediately centrifuged, and plasma was transferred to pre-prepared tubes containing acetonitrile and an internal standard of Nle-YI-(6) aminohexanoic amide, 100 g/ml in isotonic saline

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In addition, we discuss therapeutic efforts, challenges in clinical translation, and future directions, including biomarker discovery, combinatorial treatment strategies, and targeted delivery systems, to enable more effective modulation of autophagy in disease

B.DohguS.SultanaR.LynchJ