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Fluorescence ( ex = 405 and 488 nm, em = 510 nm) was measured by flow cytometry using a Becton Dickinsons FACS Aria II flow cytometer

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*Patients with bowel obstruction may have abdominal tenderness, however should not have features of guarding or rebound tenderness, unless ischaemia is developing Differential Diagnosis The differential diagnoses for a patient presenting with suspected bowel obstruction include pseudo-obstruction, paralytic ileus, toxic megacolon, or constipation Investigations Laboratory tests All patients with suspected bowel obstruction require urgent bloods on admission, as well as a Group and Save (G&S) if surgery is planned

Secondary outcome To investigate potential causes of mortality, we performed a subgroup analysis by excluding participants with specific diagnoses (supplementary Table b) prior to the index event and following them from 6 months up to 15 years