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The high-affinity interaction between streptavidin and biotin cannot be dissociated efficiently except with very harsh conditions, such as boiling in sample loading buffer for SDS-PAGE or 8M guanidineHCl, pH 1.5

[14] We need vitamins B2, B12, B6, and B9 (folate) to break down homocysteine, in order to convert it to methionine and cysteine

Therefore, combined use of GT II and GT Plus assays represents a reliable and simple approach which considerably reduced the number of ambiguous HCV-1 results and enabled a successful subtyping of 98.9% of all HCV-1 samples