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Analytes (M-CuP or D-CuP) were diluted in PBST buffer (10 mM phosphate-buffered saline, 0.05% Tween-20, pH 7.4) and injected at increasing concentrations at a flow rate of 30 L/min, with 120 s association and 180 s dissociation phases

For functional enrichment analysis, the R package gprofiler2 (v.0.2.0) was used to identify enriched functional terms (GO:MF, GO:BP, GO:CC, KEGG pathways and REACTOME pathways)

This broad gene regulatory activity establishes a cellular environment primed for regeneration, with research demonstrating that GHK-Cu treatment resets aged gene expression patterns to match younger, healthier profiles

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