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Multiple dosing with escalation to a higher dose was considered inappropriate for participants in these studies given their potential poor health status

J Mol Biol (1998) 277(5):111128.10.1006/jmbi.1998.1669 44 WalshSTJevittsLMSylvesterJEKossiakoffAA

Venous blood samples were stored at 20 C in polypropylene tubes until analysed, 80 L of ethylenediaminetetraacetic acid (EDTA) plasma was used to assess cagrilintide pharmacokinetics at each timepoint, thus 16 mL EDTA plasma from each participant was used