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The synthesis process carefully manages the incorporation of alpha-aminoisobutyric acid (Aib) at the N-terminus and the D-amino acids (D-2-naphthylalanine and D-phenylalanine), which do not occur in natural proteins but are critical to the peptide's final properties

Biological analyses were performed on serum, measuring folate, vitamin B12, homocysteine, and folate receptor-alpha autoantibodies using radioimmunoassay and mass spectrometry

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Because the published experimentally determined p97 structures show clear and pronounced interactions between K251 and ATP, all simulations for this project were carried out with positively charged K251