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Collect the viral supernatant, mix with the supernatant collected by 48 h

Briefly, cells expressing the corresponding D2-like receptors were treated with the agonist for 15 min (initial treatment) to trigger desensitization and then washed three times with serum-free medium (5 min per wash) at 37 C to remove the receptor-bound agonist

[DOI] [PMC free article] [PubMed] [Google Scholar] 86.Fontaine K.R., Barofsky I., Andersen R.E., Bartlett S.J., Wiersema L., Cheskin L.J., Franckowiak S.C