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4 Materials and methods 4.1 Cell culture and in vivo experiments 4.1.1 Cell lines Four human NB cell lines were used in this paper: Be(2)-C (MNA), IMR5-/75-shMYCN (29) (MNA, doxycycline-inducible shRNA targeting MYCN, resulting in MYCN downregulation), IMR-32 (MNA), and SH-SY5Y/6TR(EU)/pTrex-Dest-30/MYCN (non-MNA, doxycycline-inducible transgene, resulting in MYCN overexpression)

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Both semaglutide and tirzepatide are medications used to manage blood sugar levels in people with type 2 diabetes, and they have both shown promising results in aiding weight loss

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