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The immunogenicity risk assessment of TPT and selected peptide-related impurities was performed using three orthogonal methods, as previously described ( in silico tools (EpiMatrix, ClustiMer, and JanusMatrix) ( in vitro human leukocyte antigen (HLA) binding assays were conducted to evaluate differences in HLA binding affinity of TPT and its impurities across common HLA-DR allele supertypes

or immune checkpoint inhibitors 158