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Cell culture and seeding for experiments NIH-3T3, U937 and neuroblastoma cells, SH-SY5Y, were obtained from ATCC and were maintained in HyQ-DMEM media with 5% Fetal Clone III, (Gibco/BRL), 50 U/ml penicillin, and 100 g/ml streptomycin in 100 mm Petri dishes

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Type 2 diabetes: Oxidative stress plays a role in diabetes, and people with type 2 diabetes have lower glutathione levels on average.[ref] Pancreatitis: Chronic pancreatitis causes oxidative stress