Human phospho-site mutant GLP-1R construct generation SNAP/FLAG-tagged human GLP-1R wild-type (WT) plasmid (Cisbio), subsequently referred to as SNAP/FLAG-hGLP-1R, was altered by site-directed mutagenesis with the PfuUltra II Fusion High-fidelity DNA Polymerase (Agilent) as per the manufacturers protocol to modify serine to alanine doublets at positions 441/442, 444/445, and 451/452 of the receptor to generate individual serine doublet mutants (referred to as S441/2A, S444/5A and S451/2A)
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doi: 10.3389/fmolb.2023.1170181 Received 20 February 2023 Accepted 28 March 2023 Published 07 April 2023 Volume 10 - 2023 Edited by Catalina Dirney Alba Soto, University of Buenos Aires, Argentina Reviewed by Jacek Mokrosinski, Novo Nordisk Research Center Indianapolis, United States Brian Hudson, University of Glasgow, United Kingdom Updates Copyright 2023 Austin and Tomas
