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Phase 2 tested 1 mg, 4 mg, 8 mg, and 12 mg

5-8 Recommended tests include: Vitamin B12 and methylmalonic acid Serum folate Ferritin, serum iron, and TIBC Serum or RBC magnesium 25-hydroxy vitamin D Serum zinc Whole blood thiamine or erythrocyte transketolase Vitamins A, E, and K1 in high-risk individuals Comprehensive metabolic panel for electrolyte status In complex or refractory cases, advanced micronutrient testing from functional laboratories may be warranted

A practical rotation schedule: abdomen during weeks 1-4, upper thigh during weeks 5-8, upper arm during weeks 9-12, then back to abdomen for maintenance

The authors specifically called for future research to measure strength, mobility, and function rather than relying primarily on lean mass